Abstract
Quantitative PCR method for 16S rRNA gene of Microlunatus phosphovorus was developed utilizing Quenching Primer PCR (QPrimer-PCR). PCR mixture and condition were optimized. Melting curve analysis and sequencing results revealed that only the target M. phosphovorus DNA were PCR amplified, without amplification of the nontarget sequences. We quantified M. phosphovorus in five laboratoryscale enhanced biological phosphorus removal (EBPR) activated sludge processes fed with different carbon sources. The abundance of M. phosphovorus varied according to type of carbon source. In addition, M phosphovorus existed in full-scale wastewater treatment plants regardless of type of treatment methods.
