Abstract
It was investigated whether GST (glutathione-S-transferasaec) t ivity in plant leaves can be used as a biomarker of air pollution or not. As the first step, effective protein extraction method and temperature of GST-activity measurement were checked. Using the determined extraction and measurement method, GST activities of Pothos (Epipremunum aureum) exposed by road-or tobacco-pollutants were analyzed. The leaves exposed by environmental tobacco smoke in about one month showed significantly high GST activities than that of the control. It indicated the possibility of GST in plant leaves as a biomarker of air pollution, but further investigation especially about influencing factors is needed.
