Characterization of Cellular Optoporation Using Nanosecond Laser and Metal Pattern for Massively Parallel Intranuclear Delivery

Abstract

To advance intranuclear delivery of human cells, ns-pulsed laser was used for optoporation. Optical transfection techniques are less-invasive, clean, and efficient, hence optoporation is chosen. After growing cells on the substrate coated with a metallic layer of Titanium at specified micro-places, those are irradiated with laser using different energies. The goal is to optimize laser parameters for the same. Viability test and delivery rate is determined for characterization. Calcein AM and PI dye is used for viability test and FITC-dextran and PI dye is used to determine delivery rate. The study finds its major application in pharmaceutical industries, customization of medicines and drug discovery.