Chenge of serum total SH(T-SH) in hemodialysis patients

Abstract

Serum albumin can be classified as either reduced albumin (Alb-SH) or oxidized albumin (Alb-SO). Alb-SH decreases with aging and in renal disease. In hemodialysis patients, Alb-SO is increased before dialysis, but during hemodialysis, Alb-SO decreases as it is converted to Alb-SH. At present, the only available assay for Alb-SH has been HPLC, thus limiting its use in medical technology. Therefore, serum total SH (T-SH) was measured by a DTNB assay instead of Alb-SH.In this study, we measured T-SH in hemodialysis patients before and after dialysis. Total protein, albumin, BUN, and creatinine were also measured and compared with T-SH. With hemodialysis, T-SH increased, while BUN and creatinine decreased. There were significant inverse correlations between changes in T-SH and BUN, and between changes in T-SH and creatinine. Our findings suggest that measurement of T-SH may be a useful parameter in patients undergoing hemodialysis. [Jpn J Physiol 54 Suppl:S249 (2004)]