Abstract
D-Allose is one of the rare saccharides, and its pharmacological activities are not well known. Our preliminary study suggested that D-allose has anti-oxidative effects, therefore, we investigate neuroprotective effects of D-Allose against ischemic insult in this study. Rats (male, wt.400-450g) were subjected to right middle cerebral artery occlusion (MCAo) for 1 hr under pentobarbital anesthesia (50 mg/kg). Rectal temperature was controlled at 37°C with a heating pad, and D-allose was intravenously infused during occlusion and further 1hr after reperfusion (100-400mg/kg). Brains were fixed, removed, and made paraffin sections 1 wk after MCAo. Sections were stained with hematoxylin and eosin, and majored infarction volume. In another set of rats, malondialdehyde (MDA) and 8-Hydroxy-deoxyguanosine (8-OHdG) were investigated 1 day after MCAo to clarify anti-oxidative effect of D-allose. Infarction volume was significantly decreased by administration of D-Allose dose-dependently compared with saline group. Both MDA and 8-OHdG were also reduced in D-Allose group. Physiological data including blood pressure and blood sugar were not influenced by D-Allose administration. Thus, the present study suggests that D-Allose has anti-oxidative effect, resulted in neuroprotection on MCAo. [Jpn J Physiol 55 Suppl:S231 (2005)]
