Abstract
We have previously showed that endogenous vasopressin increases the vasopressin (AVP) and oxytocin (OT) mRNA production in the paraventricular nucleus (PVN) and supraoptic nucleus (SON) of the hypothalamus after central salt loading in conscious rats. We reached this conclusion by exposing hybridization signals to x-ray films. However, we are also asked to confirm this results by showing the specific signals on the brain region using double labeling procedure (Fos-like immunoreactivity: FLI and mRNA). For this purpose, all hybridized sections were dipped in emulsion (Art emulsion, Fuji film) and then stored at 4°C for 14 days and 28 days for AVP and OT assays after completion of immunostaining of FLI. The distribution of the hybridization signal on the sections were similar to that of FLI in the SON. From these results, we confirmed that central vasopressin affects its own synthesis and OT synthesis in the SON. [J Physiol Sci. 2006;56 Suppl:S214]
