Abstract
Neuroblastoma is one of the most common pediatric solid tumors, and is quite conspicuous. Therefore, the therapy of neuroblastma is a critical problem for health for children. Generally, cancer cells essentially require more nutrients including serum to growth as compared to normal cell. To investigate what kind of molecular event occurs in neuroblastoma cells in response to low serum stimulation could be very useful for understanding the cancer cell and the therapy. Therefore, we have performed proteomic analysis of neuroblastoma cells, GOTO in response to from 10% to 1% serum. The cells were analyzed for differential expression by fluorescence differential two-dimensional electrophoresis (DIGE) and liquid chromatography-Tandem Mass Spectrometry (LC-MS/MS). MTT assay and trypan blue exclusion assay were also performed to check growth and viability of the cells. As a result, six proteins were identified as cell signaling regulating proteins, apoptosis-related proteins or cellular stress-related proteins. The cells simulated showed decreased viability and growth at the day 1 after stimulation, but recovery of them at second day and later. Taken together, we here describe novel six proteins, which may be involved in survival and growth in neuroblastoma cells, GOTO. [J Physiol Sci. 2006;56 Suppl:S221]
