Abstract
Resistin is a newly identified as a cysteine-rich adipose tissue-specific secretory adipokine that may link obesity and insulin resistance. We have studied the effect of resveratrol, a phytoalexne stilbene from grapes, on the regulation of resistin gene expression during 3T3-L1 adipogenesis. Resveratrol treatments attenuated adipocyte differentiation, which resulted in a dramatic decrease in resistin mRNA levels as well as its protein levels secreted into the culture medium in a dose-dependent fashion. In contrast, epigaro-catechin-3-gallate, a main polyphenoric compound of green tea leaf which has antioxidant potency same as resveratrol, did not repress resistin gene expression in the same dose-range. Transient transfection experiments with resistin promoter/luciferase reporter revealed that resveratrol reduced transcriptional activity of the resistin gene in a dose-dependent manner. These results indicate that the resistin gene expression is down-regulated by resveratrol through the reduction of gene transcription in 3T3-L1 adipocytes. [J Physiol Sci. 2007;57 Suppl:S89]
