Abstract
D-Allose, the C-3 epimer of D-glucose, is one of the rare sugars which are rare in nature. D-Allose was shown to have an inhibitory effect on cell proliferation of various human cancer cells. In particular, various lymphoma cell lines have been elucidated to have different susceptibility to D-allose. In other words, the proliferation of some human leukemia cell lines was significantly inhibited by D-allose, and some were not. In order to explain the different susceptibility, we chose 5 leukemia cell lines, MOLT-4F (T-cell lymphoblastic leukemia), IM-9 (bone marrow), HL-60 (acute promyelocytic leukemia), BALL-1 (acute lymphoblastoid leukemia) and Daudi cell (Burkitt lymphoma). Using these four cell lines, we measured the uptake of D-allose together with other monosaccharides using 14C-labeled D-glucose, D-allose, D-fructose and D-psicose. We also investigated the expression pattern of all glucose transporters (GLUT) in these cells by the real-time reverse transcription-PCR (RT-PCR) method, since GLUTs are thought to be involved in uptake of monosaccharides including D-allose. In addition to the above analyses, we analyzed the expression of several cell-cycle related proteins using Western blotting. We discuss possible mechanisms which may explain the different susceptibility to D-allose-induced inhibition of cell proliferation among various leukemia cell lines. [J Physiol Sci. 2007;57 Suppl:S123]
