Abstract
Changes in G-proteins associate with some diseases. Here we examined whether expression of Gq/11 in the brain, lung, heart, liver, kidney and small intestine alter during acute lung injury (ALI). The concentrations of Gq/11 in six organs were measured by Western blot. ALI model in rats was established by intravenous injection of oleic acid (OA, 0.2ml/kg) through tail vein. All rats were randomly divided into a vehicle injected control group (C group) and experimental groups (OA groups). OA groups included 4 sub-groups: OA30min, OA60min, OA90min, and OA120min. Blood gas indexes (pH, PaO2, PaCO2), cardiac function indexes (MAP, LVSP, ±LVdp/dtmax), hepatic function indexes (ALT, AST), renal function indexes (BUN, Cr) and enzyme indexes (MDA, LDH, CK) of plasma and organs were measured. Each group was examined by microscopy. Compared with C group, expression of Gq/11 in OA groups increased by 20-70% in all organs with time passing. PaO2, MAP, LVSP and ±LVdp/dtmax gradually decreased, BUN and Cr in the plasma notably increased. ALT, AST, MDA contents and LDH activity increased with time passing. CK activity in the plasma, brain and heart of OA groups notably increased. Histological changes were found by microscopy. We conclude that up regulation of Gq/11 protein concentration in the organs may play a role in damaged multiple organs during ALI. Change of signal transduction mediated by Gq/11 protein may take part in the development of ALI. [J Physiol Sci. 2007;57 Suppl:S127]
