Abstract
It is well known that role of GABAA-R mediated actions is important for early CNS development. The GABAA receptor-mediate actions can regulate radial migration of cortical plate cells. GAD67-GFP knock-in mouse make it possible to investigate the effects of ambient GABA because of the decreased GABA content. It is known that GABAA receptor is activated taurine as well as GABA. In this study, we investigate the influence of the ambient GABA and taurine to cortical plate cells by using GAD67-GFP knock-in mice and taurine metabolism blocker, D-cysteine sulfinate (D-CSA).The cortical plate cells which exist in ventricular zone at E14 were labeled by in utero electroporation. Three days after the electroporation, the distribution of labeled cells was analyzed. There was no significant difference in the distribution among genotypes of GAD67-GFP knock-in mice. GABAA-R mediated currents were recorded by whole-cell recording from labeled cells. Evoked GABA current had dose-dependent manner and had no differences among genotypes. Ambient taurine was decreased by the i.p. injections of D-CSA. The radial migration of cortical plate cells was facilitated in D-CSA injected homozygous GAD67-GFP knock-in mice. Thus, taurine may have supplemental effect for GABA, that could play critical protective role in pathological condition of GABAergic system. [J Physiol Sci. 2007;57 Suppl:S177]
