Abstract
JuzenTaihoTo (JTT) is well known to frequently use for the treatment of chronic diseases and anemia with favorable success. Administration of JTT into patients with gastic cancer and liver cancer is also recognized to cause decrease in cancer volume and metastasis. Our previous works clearly showed that JTT could enhance the activity of both NK and NK T cells and results in favorably modification of the clinical onditions of cancer patients. It is well accepted that several types of cytokines such as interferon (IFN)-γ and IL-12 are responsible for the activation of NK and NK T cells.The present study, therefore, was undertaken to examine the influence of JTT on cytokines, which responsible for the inhibition of cancer metastasis by using B16 melanoma/mouse system. C57BL/6 male mice were fed normal mouse chow contained 1% JTT for three weeks.These mice were injected intravenously with 2×105 B16 melanoma cells on 8th of JTT administration. Metastasis of B16 in lung tissues were examined two weeks later.This suppressive activity of JTT on metastasis was abrogated by the pretreatment of mice with Amrinone or anti-IFN-γ antibody. In Amrinone treated group, there are many B16 metastasis in lung compared with anti-IFN-γ antibody treated group.These results strongly suggest that JTT enhances these cytokines and results in inhibition of cancer metastasis in vivo. [J Physiol Sci. 2007;57 Suppl:S243]
