Protein therapy by direct protein transduction into the brain

Masanobu Maeda; Hidefumi Waki; Akira Kohsaka; Kazunori Yukawa; Takeshi Nakamura; Ken Kohda; kyoko Owada-Makabe; Yuji Tsubota; Sabine Gouraud; He Cui; Toshiya Yamazaki; Miwa Takagishi; Eliusur Bhuiyan; Atsutoshi Hatada

doi:10.14849/psjproc.2008.0.020.1

Proceedings of Annual Meeting of the Physiological Society of Japan

Session ID : 1S-11D-2

DOI https://doi.org/10.14849/psjproc.2008.0.020.1

Conference information

Host: The Physiological Society of Japan

Protein therapy by direct protein transduction into the brain

*Masanobu Maeda, Hidefumi Waki, Akira Kohsaka, Kazunori Yukawa, Takeshi Nakamura, Ken Kohda, kyoko Owada-Makabe, Yuji Tsubota, Sabine Gouraud, He Cui, Toshiya Yamazaki, Miwa Takagishi, Eliusur Bhuiyan, Atsutoshi Hatada

Author information

*Masanobu Maeda
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Hidefumi Waki
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Akira Kohsaka
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Kazunori Yukawa
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Takeshi Nakamura
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Ken Kohda
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
kyoko Owada-Makabe
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Yuji Tsubota
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Sabine Gouraud
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
He Cui
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Toshiya Yamazaki
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Miwa Takagishi
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Eliusur Bhuiyan
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan
Atsutoshi Hatada
Dept. Physiol., Wakayama Med. Univ. Sch. Med., Wakayama, Japan

Keywords: protein transduction, protein therapy, nitric oxide synthase, nucleus tractus solitarius, cardiovascular center

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Abstract

We succeeded in attemptting targeted, direct in vivo protein transduction by microinjecting β-galactosidase (β-gal) with hemagglutinating virus of Japan envelope (HVJ-E) vector into the rat nucleus tractus solitarius (NTS) (Neurosci Letts, 378; 18-21, 2005). In this study, we investigated changes in blood pressure (BP) by direct in vivo nNOS (neuronal nitric oxide synthase) protein transduction into the rat NTS in order to examine whether the direct in vivo transducted protein in the brain has a function. The BP at 45 min to 3 hr after nNOS microinjection was significantly lower than that of control. Our findings suggest that direct in vivo protein transduction into specific restricted brain areas may have a function. The type of targeted delivery system we present may have wide applications in the administration of therapeutic proteins to the central nervous system. [J Physiol Sci. 2008;58 Suppl:S20]

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