Abstract
We study to evaluate the effects of iron-induced oxidative stress and the protective effects of saponin against oxidative damage on rheological properties of erythrocytes. Human erythrocytes were incubated for 1 hour at 37°C with 0-1 mM FeSO4 in the presence of ascorbate. Thiobarbituric acid reactive substances assay was employed to estimate lipid peroxidation. A cone-plate viscometer, and high-shear rheoscope were used to evaluate the rheological parameters in Fe2+/ascorbate-treated erythrocytes. 1) The incubation of erythrocytes with free iron caused a dose-dependent increase in lipid peroxidation, 2) Fe2+/ascorbate treatment impaired erythrocyte deformability and erythrocyte suspension viscosity. 3) Saponin partially prevented Fe-ascorbate-induced deformability impairment and decrease viscosity of erythrocyte suspensions. Sapoin is efficacious in protecting erythrocyte's against iron-mediated oxidative injury, which can be attributed to its potent reductant and radical scavenging abilities. [J Physiol Sci. 2008;58 Suppl:S212]
