Development of Accumulation Voltammetry and Electrochemical Binding Assay using Labelled Ligands

Abstract

In this review, a summary of electrochemical studies by our research group is described. These studies started from the research of anodic stripping voltammetry using a hanging mercury drop electrode, followed by adsorptive stripping voltammetry at the modified carbon paste electrode. The preparation of ligand labeled with an electroactive compound enabled the electrochemical binding assay using avidin-biotin and lectin-sugar interactions. This method was applied to the screening of endocrine disrupting chemicals. The electrochemical detection at the immobilized DNA and CNT electrodes was also investigated.