Award Review Articles, 2019 Shikata Medal
Determination of Bioactive Compounds by Highly Sensitive Electrochemical Detection in Liquid Chromatography
2020 Volume 66 Issue 1 Pages 23-30
Details
2020 Volume 66 Issue 1 Pages 23-30
Determinations of bioactive compounds were carried out by various electrochemical detections (ECDs) hyphenated with high-performance liquid chromatography (HPLC-ECD) systems. An HPLC-ECD using a capillary column (0.2 mm i.d.) achieved determinations of catechins at femtogram levels. A three-channels HPLC-ECD using a column switching technique provided to the simultaneous determination of caffeoylquinic acids and flavonoids in Chrysanthemum morifolium flowers. HPLC-ECD systems by the means of reduction of 3,5-di-tert-butyl-1,2- benzoquinone and oxidation of trolox were developed to determine polyunsaturated fatty acids and theophylline in plasma, respectively. These HPLC-ECD systems with sensitivity and precision produced a practically useful method for the analyses of biological and herbal medicine samples.
