Identification of Iron-Sulfur Oxidizing Bacteria and Properties of Iron Oxidizing Bacteria in Metal mine Water (I)

Abstract

The important results obtained on the identification of iron-sulfur oxdidizing bacteria and the properties of iron oxidizing bacteria in metal mine water were as follows:
(1) The species of chemosynthetic iron-sulfur oxidizing bacteria have been isolated from the mine water from Komagi Mine. These species are iron-oxidizing bacteria (Ferrobacillus ferrooxidans), and sulfur-oxidizing bacterea (Thiobacillus thiooxidans and Thiobacillus concretivorus), but thiosulfate-oxidizing bacteira (Thiobacillus ferrooxidans) were not certified to exist in the mine water.
(2) Iron-oxidizing bacteria grow rapidly in the basal salts of the 9K medium at a concentration of about 8000 ppm. Fe²⁺.
(3) The optimum temperature for growth of Ferrobacillus ferrooxidans is the range 20 to 30°C, and accelerated by Ferrobacillus ferrooxidans, ferrous sulfate in the 9K medium is rapidly oxidized to the ferric sulfate. At temperatures of 40 and 50°C respectively, these bacteria are not found to exist, and at 15 and 7°C the bacterial growth is very strongly inhibited.
(4) The bacterial action is most pronounced in media having pH of between 1.6 and 3.0 and in 9k media containing 200-600 ppm. N.