1997 Volume 58 Issue 2 Pages 301-308
The expression of p53 mutation and its association with nuclear DNA ploidy patterns and clinicopathological findings were studied incolorectal adenomas and cancers. The subjects were 81 cases of colorectal cancer and 19 cases of colorectal adenoma, in which the p53 gene mutation was measured by RT-PCR-SSCP or flowcytometry. There were 4 cases of mild atypia, 8 cases of moderate, and 7 cases of severe in the colorectal adenomas, and 21 casee of Dukes's classification A, 19 cases of B, 24 cases of C and 17 cases of D in the colorectal cancers.
In DNA ploidy pattern, there were 39 (48.1%) diploidy cancers and 42 (51.9%) aneuploidy cancers, significant relations to pathological features including depth of invasion, lymph node metastasis nad stage (p<0.05) were noted in the aneuploid cases. No p53 gene mutation was detected in mild and moderate atypia adenomas, but it was detected in a severe atypia adenoma (1/7). As to the cancers, p53 gene mutation was detected in two out of five focal cancers (40.0%), and in 46 out of all 81 colorectal cancers (56.8%). There was no relation to pathological findings. It was inferred that p53 gene mutation might participate with carcinogenesis. In the correlation with DNA ploidy patterns, p53 gene mutation occurred in 17 out of 39 diploid cases (43.6%) and in 29 out 42 aneuploid cases (67.7%). And p53 gene mutation was detected in nine out of ten recurrent cases. It was suggested that the expression of p53 mutation might cause DNA abnormality and might be an important factor for recurrence.
