Globulin synthesizing capacity of human peripheral lymphocytes

—in vitro measurement using ⁷⁵Se-selenomethionine—

Abstract

Human peripheral lymphocytes were cultured for 96 hours in the medium added ⁷⁵Se-selenomethionine. Incorporation of ⁷⁵Se-selenomethionine into globulin was assayed by well type scintillation counter. Phytohemagglutinin (PHA) reactivity of lymphocytes was represented as the ratio of the PHA stimulated lymphocytes to that of the unstimulated ones. The lymphocytes of healthy adult were stimulated by PHA and globulin synthesis was activated. But PHA reactivity of lymphocytes was suppressed in the patients with Hodgkin's disease, CLL, SLE, dermatomyositis, autoimmune hemolytic anemia, uremia, infectious mononucleosis and hypoplastic anemia.