Abstract
The staining of neutrophil alkaline phosphatase (NAP). periodic acid Schiff (PAS) and lactic dehydrogenase (LDH) on neutrophils, lymphocytes and leukemic cells were carried out and searched for the relation of their scoring and serum LDH activity.
1) In AML there were two groups of NAP score, namely low and high score groups. The former showed in general fairly clinical course but the latter did not so good as the former except a few case dropped down to normal score range, and also, so called atypical aplastic anemia showed high score dropped down to normal range.
2) In spite of high or low serum LDH activity, blood cell LDH score showed broad distribution. In polycythemia vera N-, L-PAS score and N-, L-LDH score were all high but were low in CML. In AML there was reverse change of N-PAS score and N-LDH score, even in remission low N-PAS score and high N-LDH score in many cases were observed. High N-, L-LDH score were seen in aplastic anemia.
3) There was reverse correlation in LC-PAS score and LC-LDH score, but that did not be observed in N-PAS score and N-LDH score and also in L-PAS score and L-LDH score. This seemed to be cellular specific characteristics which the glycolysis, respiration and LDH isozyme pattern were different in each type of cells.
