Abstract
Determination of Total Iron Binding Capacity (TIBC) using Res-O-Mat Fe kit [TIBC] offered by Daiichi Radioisotope Laboratories, Ltd. was investigated. This kit method is to determine the total iron binding capacity of transferrin by counting the radioactivity of 59Fe which was bound to transferrin after removing the serum iron from the transferrin by adding 0.6% citric acid. About 95% of unbounded 59Fe was adsorbed by Resin Strip (Amberlite IRA 400) after 90 minutes incubation, and this method is very simple and sufficient for clinical use. TIBC value determined by this method well agreed with the value of (UIBC+SI) determined by the other method. Denaturation of transferrin due to change of serum pH was investigated by Ouchterlony's method and immunoelectrophoresis, but no change of transferrin was observed. Tf value determined by immunodiffusion method showed fairly good correlation with TIBC value. SI value determined by colorimetry also well agreed with the value of (TIBC-UIBC). This kit method is found to be simple and useful in clinical application.
