Abstract
Since the introduction of Naphthol-AS-D-chloroacetate esterase staining as a selective staining for neutrophils by Moloney, it has been increasingly attaining high evaluation. Its stainability for other blood cells, however, is inconsistent. An attempt, therefore, was made to elucidate the staining pattern for blood cells and to apply the result to the cytochemical differenciation between acute myelogenous leukemia and monocytic leukemia. Naphthol-AS-D-chloroacetate was employed as substrate combined with Fast Blue BB as coupler. The results were as follows: 1. Almost all blood cells can be stained with characteristic staining pattern for each cell type. 2. Particularly neutrophils and monocytes have their own staining pattern, which can be used as a staining distinguishing between neutrophils and monocytes. 3. Differentiation between acute myelogenous leukemia and monocytic leukemia is feasible. 4. This staining is so stable that neutrophils and monocytes can be identified on old smear preparation.
