Abstract
Cytogenetic study on bone marrow cells of 15 patients with chronic myelogenous leukemia, using banding technique was performed.
Fourteen patients showed Ph1 positive and one did Ph1 negative. All patients with positive Ph1 revealed translocation between chromosome No. 22 and No. 9, except for one who showed between chromosome No. 22 and No. 17 which was unusual. The latter patient, a 17-year-old male, was diagnosed C. M. L. at 12 years of age and took a typical clinical course of adult type of C. M. L.
Among 4 patients developed acute crisis, two showed clonal evolution. Their karyotypes were 47, XY, +8, t(9 q+; 22 q-) and 50, XYY, +8, +19, +22q-, t (9q+; 22q-), respectively. One patient, a 47-year-old male, showed abnormal clone in 70% of the metaphases two years before occurrence of acute crisis and died a month later. The other patient, a 27-year-old male, showed at the time of occurrence of acute crisis and died two months later. Double Ph1 and double Y chromosomes were formed in 100% of his marrow cells. Double Ph1 chromosomes appeared to be same in morphology and only one pair of chromosome No. 9 presented an existence of dull extra segment. Peripheral lymphocytes and skin fibroblasts at the patient's terminal stage showed normal karyogram.
Both cases had chromosome No. 8 trisomy. These findings indicated that the occurrence of aneuploidy was due to non-disjunction and might be related with a condition of acute crisis of. C. M. L.
