Abstract
Capacity of in vitro colony formation in patients with CML and cytochemical studies of colonies were examined.
Peripheral leucocytes of 4 patients and bone marrow cells of 9 patients were assayed by the method of Robinson and Pike.
Results obtained were followings.
1) Peripheral leucocytes of normal individuals formed no colonies, but many colonies were formed in 2 of 4 patients.
2) Bone marrow of patients formed fewer colonies than normal individuals.
3) Peripheral leucocytes and bone marrow of patients with CML in blastic phase formed few colonies.
4) Number of colonies of bone marrow tended slightly to increase after splenectomy in 3 patients.
5) Alkaline phosphatase activity of mature granulocytes in colonies of bone marrow was elevated in 1/4 patients.
