Abstract
The authors measured the human plasma kallikrein (S) kallikreinogen (K) and inhibitor (I) by R. W. Colman's method, and further evaluated the results to compare with that of the method using chromogenic synthetic substrate S 2302 by AB KABI.
Normal range by Colman's method was 11.4±3.0 μM/ml. h for S, 112.0±20.0 μM/ml. h for K and 1.11±0.14U for I. The relation of TAME hydrolysis μM/ml.h equals 4/3·103. OD/min. at 405 nm by S2302 method was obtained. S 2302 was thought to be accurate to measure the plasma kallikrein but not to be sensitive to inhibitor.
The results on plasma samples from 79 cases with various disorder were discussed. They revealed quite interesting and rewarding data for evaluation of pathophysiological role of kallikrein system in various disorders, especially in the intravascular coagulation and fibrinolysis syndrome.
