Abstract
Colony forming cells (CFCs) in children with acute leukemia were assayed using methylcellulose clonal cell culture. Marrow from children with acute leukemia formed no colonies or very small numbers of colonies in onset or relapse, small numbers of colonies in partial remission and normal numbers in complete remission.
Blast cell colonies were formed by blood containing 81 percent leukemic cells in a child with AML. Blast cell colonies were observed not only by using PHA stimulated normal or leukemic leukocyte conditioned medium but also by using human placental conditioned medium.
Erythroid colonies in children with acute leukemia were also assayed using methylcellulose clonal cell culture. In culture of marrow, the sum total of erythroid colonies scored at day 7∼8 were none or markedly decreased in onset or relapse, decreased in partial remission, and within normal range in complete remission.
