1985 Volume 26 Issue 3 Pages 349-356
To investigate whether LTB4 also stimulates the monocyte (MON) chemotactic response, human MONs and polymorphonuclear leukocytes (PMNs) were tested for both the chemotactic and the chemokinetic responses for LTB4, by the agarose plate method, and compared with 3 other chemotactic factors; Zymosan activated serum (ZAS), bacterial culture filtrate (BCF) and formyl-methionyl-phenylalanine (FMP). 1) LTB4 stimulated both chemotaxis and chemokinesis of PMNs: Chemotactic Index (C.T.I.=chemotaxis/random mobility (RM)): FMP 2.83>ZAS 2.22>LTB4 2.20>BCF 1.33; Chemokinetic Index (C.K.I.=chemokinesis/RM): LTB4 1.88, FMP 1.78. 2) LTB4 revealed to stimulate a potent chemotaxis for MONs. C.T.I. 4h (18h): FMP 2.25 (1.75)>ZAS 1.62 (1.61)>LTB4 1.59 (1.58)>BCF 1.19 (1.29). 3) But LTB4 did not stimulate MON chemokinesis at the concentration of 20 ng/ml. C.K.I. 4h (18h): LTB4 0.71 (0.76), FMP n.d. (1.61). As the migration speed is much slower than PMN, the MON chemotactic deactivation may have occured during the long incubation period. Conclusions: LTB4 revealed to have a potent chemotactic effect for MONs, which play an important role in the late stage of inflammation, and in immune reaction system as antigen presenting cell, regulatory cell and effector cell as transformed into macrophages.
