1985 Volume 26 Issue 9 Pages 1443-1450
Differences in cellular proteins were analyzed by two-dimensional gel electrophoresis (O'Farrell) between lymphoblasts of acute lymphocytic leukemia (ALL, N=4) and normal lymphocytes (N=4). Approximately 240 spots were detected in the gel with normal lymphocyte lysate samples and 350 in lymphoblast cell lysate samples. However, 3 spots in the normal samples and 5 spots in the lymphoblastic cell samples, especially in the onset stage, were missing some of cases. Seventy-one spots, which were stained dark and large in size (major spots), were found in normal lymphocytes and lymphoblastic cell samples. Among them, 5 spots were characteristic of normal lymphocyte samples and 9 spots lymphoblastic samples. Lymphocytes obtained from patients in complete remission still retained some of the leukemic modifications in the cellular proteins. There is a possibility that some of these spots are related to the leukemic change itself.
