1986 Volume 27 Issue 5 Pages 738-746
Expression of common ALL antigen (CALLA) on the DNA stemlines of leukemic marrow blasts in 21 children with common acute lymphoblastic leukemia (ALL) was quantitatively determined by flow cytometric analysis of J-5 monoclonal antibody binding. Mean amounts of CALLA per cell widely ranged among the patients. The cells at S phase showed higher mean amounts of CALLA than the cells at G0/G1 phase, whereas the density of CALLA per unit of cell surface area in the cells at S phase was lower than in the cells at G0/G1 phase. Subpopulations of CALLA-positive leukemic cells and CALLA-negative normal marrow cells at a given DNA stemline were easily discriminated from the findings of two and/or three dimentional diagrams of CALLA-DNA. No significant relationships of various patient's features (sex, age, DNA ploidy and surface markers) to CALLA amounts were observed. Children who remained disease-free had significantly higher amounts of CALLA for G0/G1 phase cells than children who relapsed.
These findings showed that CALLA amounts for leukemic blasts might be valuable to predict treatment response of children with common ALL.
