Abstract
The platelet injury during storage under various conditions was examined using electron microscopy, SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and high resolution two-dimensional gel electrophoresis. The platelet damages were not remarkable in agitation at room temperature and no agitation at 4C on the analysis of these methods. On the other hand, the platelets stored in no agitation at room temperature and agitation in Tris-saline buffer showed marked morphological changes and appearance of four new bands (Mr=200Kd, 140Kd, 100Kd, 91Kd) on SDS-PAGE and a 140Kd peptide by the side of Gp I b/II a on two dimensional electrophoresis.
These results indicate that a suitable agitation and presence of plasma are important for the platelet preservation. Furthermore, this study also showed that storage of platelets at 4C was no notable change in the morphological and electrophoretic studies.
