2016 Volume 63 Issue 1 Pages 18-23
Bordetella petrii strain KC42 isolated from soil in Miyazaki prefecture was shown to be a microorganism with methylation and removal activities of inorganic arsenic. Arsenic methylation using intracellular extracts from strain KC42 at pH 6.5 and 35°C for 2 h yielded 35.9% methylated organic arsenic compound and 10.8% trimethylarsenic compound (TMAC). As a result of the analysis of TMAC produced under these conditions, TMAC was identified by trimethylarsine oxide and arsenobetaine. In experiments of arsenic adsorption by dried cells of strain KC42, the ratio of As(III) adsorption by strain KC42 was 99.4% in solutions containing 0.2 mg As/L at pH 7.0 and 30°C for 2 h. The maximum adsorption value was 0.179 mg As/g dw at an initial concentration of 10.0 mg As/L As(III). These results suggest that strain KC42 may be utilized for bioremediation
