Quantitative and Qualitative Research of Surface Bacteria on the Floor of Station Restrooms with Two Types of Cleaning Method

Abstract

There are two types of method for cleaning station restrooms. One method uses water for cleaning floors, whereas the second does not. This study describes quantitative and qualitative research into the surface bacteria found on station restroom floors where these two cleaning methods were used. Samples for analysis were collected from five positions on the floor of each restroom in each railway station investigated. Samples were treated using a conventional culture method to measure the concentrations of surface bacteria on each restroom floor. Samples were then analyzed with bacterial 16S rRNA genes to analysis the microbiomes on these restroom floors. Results showed that the restrooms cleaned without water had lower concentrations of bacteria, than the microbiomes from restroom floors cleaned with water.