Abstract
We investigated the taxonomic diversity of Nocardia strains freshly isolated from six sediment samples taken from Lake Suwa, Nagano Prefecture, and from the moat surrounding Takeda Shrine, Yamanashi Prefecture, Japan. A restriction fragment length polymorphism (RFLP) analysis of 16S rDNA was used for species-level identification. Of the 25 test isolates, 23 strains were identified as N. asteroides, N. carnea, N. flavorosea, N. nova, N. uniformis, or identified as belonging to the N. africana-N. veterana group. Based on RFLP patterns, the remaining two strains were differentiated from the known Nocardia species.
