Actinomycetologica
Online ISSN : 1881-6371
Print ISSN : 0914-5818
ISSN-L : 0914-5818
Original Articles
Isolation of Gram-positive Bacteria with High G+C from Inside Soil Aggregates
A. MatsumotoY. TakahashiY. IwaiS. Omura
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2006 Volume 20 Issue 2 Pages 30-34

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Abstract

In the present study, we tried to isolate bacterial strains from inside soil aggregates as generally microbial flora inside differs from those outside. In order to effectively isolate microorganisms from inside the soil aggregates, we washed the surface with electrolyzed NaCl solution, which possesses bactericidal activity, then fractured them with an ultrasonic processor. Gram-positive bacteria with high G+C isolated from the inside of soil aggregates were then compared with those isolated from outside on the basis of their 16S rDNA partial sequences. The results showed that the two sets of isolates differ.

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© 2006 The Society for Actinomycetes Japan
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