Two New Species in the Genus Actinomadura: A. glomerata sp. nov., and A. longicatena sp. nov.

Abstract

Taxonomy of two new actinomycete strains, which were isolated from a decayed leaf and a root of herbaceous plant, was studied. The organisms had branched substrate mycelia and chains of arthrospores on aerial mycelia. Cell walls of both strains contained meso-diaminopimelic acid and whole-cells contained madurose as a diagnostic sugar. Major menaquinone was MK-9(H₆), and major fatty acids were hexadecanoic acid, 14-methylpentadecanoic acid, octadecenoic acid, and 10-methyloctadecanoic acid. Diphosphatidylglycerol, phosphatidylinositol, and phosphatidylethanolamine were detected as major phospholipid components. These morphological and chemotaxonomic features suggested that the isolates related to the genus Actinomadura, although most of Actinomadura species contain no major amount of phosphatidylethanolamine. Furthermore, comparison of the 16S rRNA genes allocates the two isolates to the genus Actinomadura. From their morphological, physiological, biochemical characteristics and DNA-DNA hybridization data, the two isolates represented two new species in the genus Actinomadura, for which we propose A. glomerata sp. nov. (type strain, I-226=JCM 9376), and A. longicatena sp. nov. (type strain, I-497=JCM 9377).