Phylogenetic relationships of Nocardiopsis species based on partial gyrB and 16S rRNA gene sequences

Abstract

Partial nucleotide sequences of the gyrB gene were determined for members of the genus Nocardiopsis and subjected to phylogenetic analysis. Interspecies DNA similarity values of partial gyrB gene sequences ranged from 79.9 to 99.7% among the Nocardiopsis species. The average similarity of the gyrB gene (87.7%) was significantly less than that of the 16S rRNA gene (96.65%), indicating a high discriminatory power of the gyrB gene. The topology of neighbor-joining, maximum likelihood and maximum parsimony phylogenetic trees based on nucleic acid sequences and protein sequences of gyrB gene were reconstructed. Compared with the 16S rRNA gene dendrogram, the gyrB gene provided more information for understanding the genetic relationships among Nocardiopsis species. Using the information derived from 16S rRNA and gyrB genes, a more comprehensive understanding of the evolutionary history of Nocardiopsis species was obtained.