1997 Volume 28 Issue 3 Pages 143-148
Pollen tube cultivated in vitro is considered to be a simple and useful method qualifying the maturity and quality of composts. However, necessary works to be done previously are to determine the suitable conditions of concen-trations of agar, sucrose and boron, as well as pH values, temperatures and culture times for each kind of the pollen used.
The obtained results of the experiments on the Lycopersion esculentum Mill., Cucurbita moschata Duch, Zea Mays L., Solanum melongena L., and Stewartia pseudo-Camellia Maxim. showed as follows:
1) The suitable concentrations of agar and sucrose for the pollen culture of the Solanum melongena L. and Stewartia pseudo-Camellia Maxim. were 0.8% and 10.0%, respectively.
2) The suitable pH levels were 6.0-6.5 and 6.5-7.0 for the Solanum and the Camellia, respectively.
3) The suitable concentration range of boron for the Solanum was from 8 to 24ppm, the one for the Camellia was 4 to 16 ppm. Temperature of about 25°C to 30°Cwas enough for both pollen kinds, and the culture times were 16h. for the Solanum and 8h. for the Camellia.
