Abstract
Under artificial environment, such as plant factory, screening environmental factors which affect strongly to the plants is expected to be useful. To search a screening method of the environmental factors, two-dimensional electrophoresis of leaf proteins was applied to analyze the different environmental conditions as a trial. Proteins were extracted from the leaves of petunia plants grown under two different kinds of light quality; white and yellow lights. Maps of the two dimensional electrophoresis showed several different printings between white and yellow lights. The result showed the possibility that two dimensional electrophoresis of leaf proteins could be one of the screening methods for the environmental factors. For the correct comparison of maps, standard procedures to extract, separate and analyze multi protein samples under the same experimental condition should be needed.