Abstract
The isolation of Na-phytate-decomposing microorganisms was attempted from solid farmyard manure using the culture medium, which contained Na-phytate as a sole source of P, and the stimulation of phytate P was also attempted by inoculating the fungal isolates in pig farmyard manure. The following results were obtained.
1) Numerous Na-phytate-decomposing microorganisms were obtained from cow farmyard manure. Among them fungal isolates could more vigorously decompose Na-phytate than bacterial and actinomycetal isolates.
2) Using the fungal isolates of high ability of Na-phytate decomposition, their ability to decompose Ca-phytate, Zn-phytate and Cu-phytate were examined. Although all isolates could decompose Ca-phytate, only fungal isolates could decompose Zn-phytate and Cu-phytate.
3) The fungal isolates of high ability to decompose Zn-phytate and Cu-phytate were inoculated to pig farmyard manure, and were incubated at 25°C for 90 days. In comparison with the uninoculated manure, inorganic P was increase by 17% and phytate P was decreased by 21% in the inoculated manure.
