Abstract
Although the restricted dynamic range of conventional proteomic technology using two-dimensional gels and mass spectrometry has limited applicability (i.e. plasma proteome), our recent studies of proteomics technologies using electrophoresis to discover and identify new biomarkers are summarized. Low-abundance proteins are rarely seen on traditional two-dimensional gel electrophoresis (2-DE) analysis of plasma because of the highly abundant soluble proteins such as albumin and globulin in plasma. In this study, serum proteins can be separated into two fractions by the Con A-Sepharose column (retained fraction was enriched in N-glycosylated proteins), which is used for the exclusion of albumin and is a simple method to analyze proteins in plasma. The % area of each protein band was compared between the different groups and proteins in each band were identified using LC/MS/MS. In addition, we also summarized recent results from focused brain proteomics to identify proteins associated with dysmnesia using 2-DE. Neuronal protein expression in viral infected mice was compared with that in non-infected mice using the Image Master 2D. We detected approximately 700 protein spots, of which approximately 40 spots were distinguishable between non-infected and virus-infected mice. This proteomic analysis is a useful tool for identifying proteins in mouse diseases model (especially using KO or TG mice).
