Abstract
Horse γ-globulin (2g) obtained from the antiserum specific to human α-fetoprotein was coupled to Sepharose 4B (settled volume 50ml) activated by the BrCN method of Porath et al.
Ascitic fluid of a hepatoma patient was applied to the antibody column and α-fetoprotein was specifically adsorbed on the column. The adsorbed protein (50mg) was dissociated and recovered with an acidic buffer, 0.1M glycine-HCl, pH 2.8. The column could be used repeatedly more than 10 times without any loss of the adsorbing capacity. The preparation showed high homogeneity by electrophoretic and immunological analyses.
Crystalline α-fetoprotein was obtained from the purified material in an ammonium sulfate solution.
