Abstract
In order to clarify some relations between serum constituents and Takata's reaction, experiments, were carried out employing sera of different patients and various serum fractions prepared by electrophoretic method and Na2SO4-salting -out method. Summary of the results obtained are as follows.
1. In the course of titration with Takata's reagent, γ-globulin required the least volume of the reagent to cause a precipitation, then α-, β-globulin followed next, and albumin consumed the most quantity of the reagent in comparison with the other preceeding two fractions.
2. Generally, the more γ-globulin and the less albumin the sernm contains, the more they inclined to present stronger reaction in the Takata's reaction.
3. Ratio of protein constituents in serum was considered to be one of the governing factors for the out-come of Takata's reaction, but the reaction was not influenced by the protein concentration of serum.
4. Results of Takata-Domoto's reaction were influenced by both the ratio of protein constituents and the protein concentration of serum.
5. Colour changed from pink to violet with increase in the serum protein concentration in the course of Tadata's reaction. Pink colour more or less faded away with the time. The protain concentration in serum at the turning point from pink to violet stayed around 0.1% regardless of protein constituents in serum.
6. Ratio of protein constituents did not always endorse the out-come of Takata's reaction when the serum of some patient were employed. This exceptional phenomena are presumed to be caused by the factors other than the ratio of serum protein constituents.
7. Particles precipitated in the serum containing a large quantity or γ-globulin was gross in size, however, the other serum proteins produced fine particle when precipitated.
