Cellulose acetate electrophoresis using a new Separax-S membrane and its normal serum protein fractional values in adults and children

Abstract

An improved new membrane, Separax-S, was studied for its routine application of the serum protein fractionation. In comparison to the conventional Separax membrane, Separax-S has less endoosmotic effect and gives more reproducible and clear protein sepration. The application point of the serum now locates on the beta fraction, and the linear beta-lipoprotein band is less apparent. The fractional values of the serum protein are not significantly different from those of the conventional membrane, except that the beta fraction is slightly higher with the new membrane, Separax-S. Glycoproteins, lipoproteins, and isoenzymes of lactate dehyrogenase and alkaline phosphatase could be separated sufficiently with the new membrane.
The normal serum protein fractional values in both adults and children are reported by using the new Separax-S membrane with the standard methodology.