Studies on LDH isozyme in bovine serum

Abstract

By using the simple thin layer-PAGE, lactate dehydrogenase (LDH) isozymes in bovine serum were determined during the development of fetal and normal pregnant stage, and the isozymes in serum from patient with downer cow syndrom were determined.
All five forms of LDH isozymes were obserbed in bovine fetal serum. The total LDH activity and the fast-moving isozyme LDH₁ increased in bovine fetal serum during development.
In the pregnant maternal serum, LDH₁, LDH₂ and LDH₃ were detected, while LDH₄ and LDH₅ were not detected, in which LDH₁ had the greatest percentage. There were no changes of LDH isozyme pattern in bovine serum during the pregnancy.
The slow-moving isozyme LDH₅ and total LDH activities most increased in serum from patients with downer cow syndrom.