Abstract
Continuous gradient polyacrylamide microgel electrophoresis is one of the methods for examining a minute amount of sample. We applied this method for the investigation of proteins of cerebrospinal fluid (CSF) in normal subjects and patients suffering from neurological diseases. We could analyse the proteins of unconcentrated CSF in amount of under 1μl. The amount of albumin was calculated directly from the area under the albumin peak on the densitogram with the standard line of albumin. Twelve bands of proteins were obvious and they always appeared at the same positions on the gel in all normal CSF samples. Proteins more anodic than albumin were clearly analysed in this method as well as those more cathodic than albumin. We examined the relationship between various neurological diseases and the amount of the protein which was located at the most anodic side (band 1).
