Abstract
The microhetrogeneity of human transferrin were examined by isoelectric focusing with modified immobilized pH gradient.
Purified human diferric transferrin were separated to four bands (A: pI 5.15∼5.19, B: pI 5.26∼5.30, C: pI 5.37∼5.42, D: pI 5.50∼5.54) on immobilized gradient gel. The statement of equality between four transferrins ratios is a proportion A:B:C:D=1:3:5:1. No significant difference between the binding of each transferrin fractions to cell surface receptor was obserbed. The presence of A, B, C and D transferrin fraction in native normal serum was detected by immunofixiation test.
