Abstract
The effect of gelatin that was added into agarose gel on the separation of DNA is reported. Addition of gelatin R (pI 8.4) to agarose gel caused interference with migration of DNA fragments. Addition of modified gelatin S9, which was prepared by succinylation of the amino groups of gelatin R, resulted in appearance of molecular sieving effect and separation of DNA fragments at rates that were inversely proportional to log10 of molecular weights. The interference due to addition of gelatin R may be caused by interaction between DNA and the amino groups of gelatin. The same effect was observed for the gel made solely of gelatin R. The DNA fragments separated in gelatin R gel were easily recovered by phenol treatment and subsequent precipitation with ethanol.
