Determination of the each fractional value of normal human serum protein by electrophoresis with use of the new cellulose acetate membrane; SEPARAX-SP

Comparative studies with the conventional membranes

Abstract

The clinical reference values for serum protein fractionation were determined by mean of AES 600 (automated electrophoresis system, OLYMPUS Co., Ltd.) using a new cellulose acetate membrane “SEPARAX-SP.” All of the fraction values obtained using the new membrane were similar to those obtained using the Sartorius membrane in which electroendosmosis was fairly less. than in the case of SEPARAX, however, the α₂-fraction value measured by the SEPARAX-SP was higher than in the case of the Sartorius membrane, because β-lipoprotein migrated as the α₂-fraction.
Separation properties varied according to their adsorptive activities with respect to the serum proteins, as revealed by an expert system consisting of quantitative and graphical analysis of digital signals from the densitometer of the AES 600.
In addition to comparison with the conventional membrane, SEPARAX and Sartorius were compared with each other.
The utility and effectiveness of a new membrane “SEPARAX-SP” can be best appreciated on the basis of our experimental results.