Protein analysis by untreated fused silica capillary electrophoresis

Abstract

Separation of 11 model proteins of various pI and MW values, and proteins in human serum, were performed by capillary electrophoresis using untreated fused silica capillary with 100mM sodium borate (pH10.0) for electrolyte and 100mM phosphoric acid (pH2.5) for washing the capillary between the run. Proteins in human serum were also separated into five fractions (albumin, α₁, α₂, β and γ-globulin) by the same conditions as above within 8min. The model proteins showed the migration time based on their pI values and the reproducibility of both the migration times and peak areas was good except for a few basic proteins.