1994 Volume 38 Issue 1 Pages 31-36
Differences between alkaline phosphatase (ALP) isozyme patterns obtained by cellulose acetate membrane (CA) and polyacrylamide disc gel (PAG) electrophoreses were compared. With the PAG, high-molecular mass ALPs can be detected more frequently than those with the conventional CA. The high-molecular mass ALP migrated to the pre-liver ALP position on the CA, but was separated into the two retained ALP fractions on the PAG: one is that detected at the top position of the stacking gel (retained 2, R2); the other is that detected at a position between the stacking and the loading gels (retained 1, R1). After treatment of serum with 2% Triton X-100, the two retained ALPs could hemogeneously migrated to the position of biliary ALP. The contents of the R1 and R2 ALPs in serum were related to pathological conditions of gastrointestinal disorders. Although the R2 ALP was frequently observed in benign hepatobiliary disorders, the R1 ALP detected more in the gallbladder and pancreas carcinomas. The retained ALPs were extracted from the PAG with 2% Triton X-100 and treated with phosphatidylinositol specific phospholipase C. The resulting aliquots were further subjected to the PAG electrophoresis for analyzing the organic origin of ALP isozymes. The results showed that the R2 ALP contained the liver, bone, and intestinal ALP(s), whereas the R1 ALP had mainly liver ALP. These findings suggest that the retained ALPs serves a useful information for the diagnosis of damaged organs in clinical practice because of the organ specificity of R1 and R2 ALPs.
